Dear friends in crystallography, I know this seems unrelated, but it really isn't ... please forgive me. We are trying to use the Avitag/BirA system to specifically biotinylate target proteins in an economical manner. Are any of you purifying the BirA enzyme in your lab for biotinylation and if so can you help me figure out the right plasmid/purification protocol? Please let me know. Thank you. Gloria
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