On Monday, 16 December 2019 09:25:59 PST Jessica Besaw wrote:
> Dear community,
>
> I am having a lot of trouble solving a protein structure. I think my
> problem may caused by incorrectly placed proteins in molecular replacement.
> I have two proteins in my asymmetric unit. It appears that one protein fits
> perfectly, and the other one has many errors. (See snapshots below). I have
> tried deleting the parts of the protein (and even the whole protein) to try
> and rebuild it in COOT, but it was a bit too difficult for me to solve.
>
> I would appreciate any and all suggestions for potential strategies moving
> forward.
>
> Other information:
> (1) 2.4 Angstrom
> (2) 99% complete
> (3) "Translational NCS may be present at a level that may complicate
> refinement"
Try turning off the translational NCS check.
We have had two structures where incorrect diagnosis of tNCS prevented
Phaser from finding the correct solution. If you are not using Phaser, the
caveat still stands in principle.
Ethan
--
Ethan A Merritt
Biomolecular Structure Center, K-428 Health Sciences Bldg
MS 357742, University of Washington, Seattle 98195-7742
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