Dear Rajnesh, I wonder about your chosen space group? I commend that you expand your diffraction data into P1, then run molecular replacement with Phaser in P1 and if a solution is determined by Phaser run the coordinates through Zanuda to determine the space group. Best wishes, John Emeritus Professor John R Helliwell DSc
> On 12 Feb 2020, at 07:23, Rajnesh Kumari Yadav <rajn...@rcb.res.in> wrote: > > Hello everyone, > > I am working on a protein which have 5 domain in it, we have its 4 domain > structure and 3.0 Angstrom data of 5 domain protein crystal. While doing the > Molecular replacement we got solution with four domain with good electron > density and space for last domain without any density. When we tried > Molecular replacement with the missing domain (modelled) only we got solution > shows density for this missing domain but not able see electron density for > rest four domain even though room for 4 domain was visible in the solution. I > need help or suggestion for this issue. > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
