Well - there is something funny about your first Rfree - it shouldnt be significantly lower than R? I suspect there is some muddle over the assignment of Rfree - one used for Phaser and a different value for REFMAC?
And of course at low resolution especiall Rfactors are very sensitive to scaling procedures.. Eleanor On Wed, 8 Apr 2020 at 14:08, Kyle Gregory < 00003632e92fcc15-dmarc-requ...@jiscmail.ac.uk> wrote: > Hello, > > I haven't seen this before but doing a round of refinment with REFMAC, > after molecular replacement with phaser, my R factor and R free have > increased? > > Also is it weird that my Rfree is smaller than my R factor? > > *Result:* > Initial Final > R factor 0.3621 0.3761 > R free 0.3108 0.4733 > Rms BondLength 0.0076 0.0057 > Rms BondAngle 1.6504 1.5193 > Rms ChirVolume 0.0600 0.0526 > My map was ok(ish) but there was indication of anistropy so I attempted to > improve it by using STARANISO and re-ran phaser with the output, and this > is the results I see following REFMAC. > > Thanks, > > Kyle > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1