Hi Mark, NEB
https://international.neb.com/products/p8112-<https://international.neb.com/products/p8112-tev-protease#Product%20Information>tev<https://international.neb.com/products/p8112-tev-protease#Product%20Information>-protease#Product%20Information<https://international.neb.com/products/p8112-tev-protease#Product%20Information> And Sigma https://www.sigmaaldrich.com/catalog/product/sigma/t4455?lang=en®ion=GB Both sell His-tagged TEV protease. Maybe suitable? HTH, Dave -- Dr David C. Briggs Senior Laboratory Research Scientist Signalling and Structural Biology Lab The Francis Crick Institute London, UK == about.me/david_briggs ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Mark J van Raaij <mjvanra...@cnb.csic.es> Sent: Thursday, July 2, 2020 10:31:37 AM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Commercial source of His-tagged protein Dear All, Wondering if anyone knows of an economical commercial source of a soluble His-tagged protein. In principle any His-tagged protein would suffice, because it's for testing coupling to a surface via the His-tag. Asking for an international collaborator, who don't have access/expertise of a molecular biology lab (except us :-). We can and will make the His-tagged proteins of interest for them, but if there is an economical and reliable commercial source, it might be worth using that for initial tests. Best wishes, Mark Mark J van Raaij Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC calle Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 Section Editor Acta Crystallographica F https://journals.iucr.org/f/<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fjournals.iucr.org%2Ff%2F&data=02%7C01%7C%7C21199949a9194fcb6b3208d81e6ac16a%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C1%7C637292791145843043&sdata=6Ny0bF1f8t0oQ5oqzmT0DNEwVqOkHCefuaQrjAbcdFs%3D&reserved=0> ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2FWA-JISC.exe%3FSUBED1%3DCCP4BB%26A%3D1&data=02%7C01%7C%7C21199949a9194fcb6b3208d81e6ac16a%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C1%7C637292791145853042&sdata=dyV7%2Fd%2BwQXd7%2FvM07LyBm9omZohMEhQ2Chy1tpaDlMg%3D&reserved=0> The Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a company registered in England and Wales no. 06885462, with its registered office at 1 Midland Road London NW1 1AT ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
