Yes, if you don’t match the inner and outer stream rates you get turbulent flow at the boundary between them and ice build up on the sample. Best wishes Elspeth
From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of Marcus Winter Sent: 03 July 2020 09:29 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] flow rate for cooling stream? Dear Patrick, Herman, Regarding the Cryostream, then referring to the Cryostream 800 brochure indicates that the N2 (gas) flow rates employed are in the range of 5 - 10 litres / minute, … and, I guess, therefore something similar for the outer dry air 'shield' stream. Obviously, it’s best to check directly with the manufacturers (Oxford Cryosystems) directly on the details. All The Best, Marcus Winter (Rigaku) -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Schreuder, Herman /DE Sent: Friday, July 03, 2020 7:44 AM To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] AW: [EXTERNAL] [ccp4bb] flow rate for cooling stream? Dear Patrick, if I recall correctly, our systems run at 10-15 ml/min (gas). I will check on Monday when I am back in the lab. The original cryostreams would run for several day's on a tank of liquid nitrogen. However, they had significant hardware to dry the nitrogen and to ensure a constant flow. Best, Herman -----Ursprüngliche Nachricht----- Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>> Im Auftrag von Patrick Loll Gesendet: Donnerstag, 2. Juli 2020 22:03 An: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Betreff: [EXTERNAL] [ccp4bb] flow rate for cooling stream? EXTERNAL : Real sender is owner-ccp...@jiscmail.ac.uk<mailto:owner-ccp...@jiscmail.ac.uk> Sorry, way off topic: Does anyone have an estimate for the flow rate one would typically use for the cold nitrogen stream passing over a protein crystal in a standard data collection? Background: Our nitrogen “generator” has gone belly-up and the vendor no longer services it, so I’m testing the feasibility of using the boil-off from a liquid nitrogen tank to provide the gas to support a short data collection (this nitrogen gas would serve as the feedstock into our helium cryostat). But I don’t know the flow rate required, so I can’t calculate if one tank has enough nitrogen to support a day or so of data collection. There are flow meters for the warm and cold stream on the nitrogen generator, but these flow meters have no apparent units anywhere on them, so I have no idea of the rate at which the gas would be consumed. Thanks for any useful tidbits. And for those of you in the US, best wishes for a happy “Holy crap, even MORE fireworks?!?!!” Day Pat --------------------------------------------------------------------------------------- Patrick J. Loll, Ph. D. Professor of Biochemistry & Molecular Biology Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St., Mailstop 497 Philadelphia, PA 19102 USA (215) 762-7706 pjl...@gmail.com<mailto:pjl...@gmail.com> pj...@drexel.edu<mailto:pj...@drexel.edu> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_WA-2DJISC.exe-3FSUBED1-3DCCP4BB-26A-3D1&d=DwIFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=VneuoA5-6DXNCKIjhZIadysng0TaNK_RNr0BvxjRo4o&s=_ddn2_elU6gq5ZhiU4pfkyTWAgVI5j3OoezaZiKUAbs&e= This message was issued to members of https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_CCP4BB&d=DwIFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=VneuoA5-6DXNCKIjhZIadysng0TaNK_RNr0BvxjRo4o&s=ODhtRaAXr7HIrYoQ7C4ZOc5egkrkE6ZSuZu3yM6I1Uw&e=, a mailing list hosted by https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk&d=DwIFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=VneuoA5-6DXNCKIjhZIadysng0TaNK_RNr0BvxjRo4o&s=asWzspAR9776AV0v6FT34hmni7fpk7sBmK7yBcwSIM4&e=, terms & conditions are available at https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_policyandsecurity_&d=DwIFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=VneuoA5-6DXNCKIjhZIadysng0TaNK_RNr0BvxjRo4o&s=MV06qT_bKqjEogZvmunm35IV9K2XImuYLUu5czNEoV4&e= ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB<http://www.jiscmail.ac.uk/CCP4BB>, a mailing list hosted by www.jiscmail.ac.uk<http://www.jiscmail.ac.uk>, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
