Dear All, I am working on a carbohydrate-binding protein, which co-crystallizes with maltose, maltotriose, maltotetraose and maltopentaose and the same can be supported by ITC experiments as well. Also, the mutant protein (X2Y) co-crystallizes with maltose, maltotriose, maltotetraose and maltopentaose, however, the binding of only maltotriose and maltotetraose could be observed through ITC. For your information, the ITC conditions are the same for all the ligands and the ligand concentration used in ITC is same as used in crystallization (100x of protein concentrations). Moreover, from structural analysis, we have observed that the binding mode of all ligands is the same.
I request your suggestion on why maltose and maltopentaose do not show any binding to the mutant protein in ITC experiments. Looking forward to suggestions. Best Regards, Monika ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
