You need to have crystal between polarizing plates. If you use only one on the top (close to your eye) and rotate filter you will “filter/polarize” all the light coming to your eye. What you need to buy is polarizing filter sheet (linear) which you place under your crystal and another one (the one that you submitted link) on the microscope (check size as you will have to secure it in order to rotate filter) The linear sheets can also be bought on amazon.
Michal On Aug 16, 2020, at 09:47, V Nagarajan <tato...@gmail.com> wrote: CAUTION: External to USask. Verify sender and use caution with links and attachments. Forward suspicious emails to phish...@usask.ca Hi, place a linear polarizer below the tray and one above it, rotating the top one until you get the desired view. V. Nagarajan JANSi On 8/16/2020 8:26 AM, Diana Tomchick wrote: It's my understanding that you have two polarizers on your polarizer-microscope--one in the base, and the one that attaches to the magnifying lens. When you rotate the one on the lens so that it is 90 degrees to the one in the base, no (or very little) light should pass through to your eyes, unless there is a crystal that plane polarizes the light at an angle that differs from the two on the microscope. What you have is just one polarizing lens. Not sure how that would work, even if it is a circular polarizer. Diana ************************************************** Diana R. Tomchick Professor Departments of Biophysics and Biochemistry UT Southwestern Medical Center 5323 Harry Hines Blvd. Rm. ND10.214A Dallas, TX 75390-8816 diana.tomch...@utsouthwestern.edu<mailto:diana.tomch...@utsouthwestern.edu> (214) 645-6383 (phone) (214) 645-6353 (fax) ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK><mailto:CCP4BB@JISCMAIL.AC.UK> on behalf of Matthias Zeug <matthias.z...@gmx.de><mailto:matthias.z...@gmx.de> Sent: Sunday, August 16, 2020 9:05 AM To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> <CCP4BB@JISCMAIL.AC.UK><mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] polarizer EXTERNAL MAIL Hi all, The polarizer-microscope in our facility is not working properly, and I have to check my plates using a standard stereo-microscope. As a workaround, I thought about buying one at Amazon, placing it on top of the plates and rotating it to still test for birefringence. The product is linked below. Does anyone have some experience with this kind of "homemade" system? And also (this might be a stupid question), does the product even work? As far as I know, the polarizers in the microscopes are linear polarizers, whereas the product linked below is a circular polarizer. I would also be happy for product recommendations (optimally available at the German Amazon). Cheers Matthias https://www.amazon.de/dp/B00XNMXYBY/ref=cm_sw_r_cp_apa_i_5YsoFbFQXTBP9 ___________________ Buchmann Institute of Molecular Life Sciences Goethe University Frankfurt ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 CAUTION: This email originated from outside UTSW. Please be cautious of links or attachments, and validate the sender's email address before replying. ________________________________ UT Southwestern Medical Center The future of medicine, today. ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
