Hi Everyone，
Well,My Research Protein is easily Dimerzation caused by Disulfide bond for many Cys.And it leads to the SEC peak too wide about 5 mL. Then I Cut N-ter 30 Amino Acids in which 5 spaced Cys,but the SEC Peak is still wide and hard to Crystallize（as the protein is very pure and clean）.Besides, I have tried different Buffer pH ＜ pI or pH ＞ pI，but the SEC peak Do not Work at all.
Anyone could offer your kindly ideas,I would thank you very much！
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