Hey Yong, Oooh, thank you - and what's more, the crystals are sapphire blue (but it's the protein that does it, not us!). The dimer affinity is not high, even with the rest of the protein included and we have never studied the long helix alone, so it might not anti-parallel dimerize at all. On the other hand, it has two somewhat flexible positions, so in the free state it might be able to find a different energy minimum. Good luck! Jon
-- Jon Hughes Sent without the use of Apple products. ---- Yong Tang schrieb ---- What a beautiful structure, Prof. Hughes - perfect crystallographic dimeric symmetry! Harder to dissect and isolate the dimerization element to my first look but I will look closer. yours, -yong On Thu, Sep 24, 2020 at 5:07 PM Yong Tang <liutan...@gmail.com<mailto:liutan...@gmail.com>> wrote: Thanks so much Jon - let me look into it. Yours, Yong On Sep 24, 2020, at 4:59 PM, Hughes, Jonathan <jon.hug...@bot3.bio.uni-giessen.de<mailto:jon.hug...@bot3.bio.uni-giessen.de>> wrote: hallo yong, the 60 Å connecting helices in 2VEA (Cph1 phytochrome) form a non-physiological antiparallel dimer (at least with the help of the attached domains).... best jon -- Professor Jon Hughes, Plant Physiology, Justus Liebig University, Gießen, Germany. email: jon.hug...@uni-giessen.de<mailto:jon.hug...@uni-giessen.de> homepage: http://www.uni-giessen.de/fbz/fb08/Inst/pflphys Sent without the use of Apple products Von: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>> Im Auftrag von Yong Tang Gesendet: Donnerstag, 24. September 2020 21:19 An: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Betreff: [ccp4bb] short anti-parallel homodimerization motifs Dear all, I'm wondering if you have personal experiences with an antiparallel homodimerization motif that I may use to add to my target protein for homodimerization. I was thinking along the lines of a helix or beta sheet (or hairpin) that is capable of antiparallel homodimerization. For the interest of minimizing the size of such a "fusion" or "tag", a "full-size" dimerization domain is less favorable. Thank you in advance for your tips. Sincerely, -yong ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
