Hi All, I have two constructs having different ori, p15ori and M13 ori, different promoters araBAD promoter and LacI, and different antibiotic resistance chloramphenicol and Ampicillin respectively. I am managed to get the transformants and getting the expected result after blunt digestion with the EcoRV enzyme. Since both the plasmids have the site for EcoRV. But the p15 ori has a low copy number that's why I am seeing the very faint band as compared to other plasmid in the sample.
So I would like to know is there any way that I can quantify the low copy number plasmid. Because I am not able to sequence it with the specific primers it could be due to its low concentration. Please advise. Thank you. -- Dr. Anamika Singh Post-Doctoral Fellow Silberman Institute of Life Sciences Hebrew University of Jerusalem, Israel No: 054-294-8036 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
