Dear Bulletin Board, Sorry for the slightly off-topic question, but we are struggling with a receptor domain that expresses well as a fusion protein, but gets lost the moment it is cleaved from the fusion partner. It could be that the receptor domain is not or misfolded, but it could also be a solubility problem.
I have seen some crystal structures of fusion proteins with MBP and for membrane proteins, T4-lysozyme fusions are often crystallized. What is your experience? Would it be worth trying to express and crystallize a fusion protein, or would it be better to look for other constructs, e.g. to include more receptor domains? Thank you very much for your advice! Herman ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
