Dear Eleanor, Thank you so much. As you said, it is a problem that the number of reflections estimated by FreeR set is different from an actual that of reflections because of the low resolution discrepancy. Moreover, twin refinement includes a problem about some overlapping of diffraction spot.
But fortunately, I solved it. I just add twin tolerance parameter to refmac. In my case, parameters of L and H test slightly increase and the space group is P21, suggesting that it seems not to a merohedral twinning but a pseudo-merohedral twinning. In the case of pseudo merohedral, higher tolerance sometimes causes resolution stretch problem. After the refinement, resolution range and electron density map look fine so that I concluded that's the reason. Regards, Yuji ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
