Hello, just to double check, I assume that you input the correctly oriented and positioned structure of each domain from successive runs as a 'fixed' model? Cheers, Jon.C.
Sent from ProtonMail mobile -------- Original Message -------- On 11 May 2021, 02:53, Lande wrote: > Hi everyone, I started my work on data processing in protein crystallography > about half year ago and have done a fair number of “easy” structures. > Recently, I processed a tutorial structure containing 3 different domains and > two chains of them (in particular, it’s “ mth685, high resolution data” from > ccp4 legacy tutorial), but it was failed during MR process. > > According to this tutorial, I did MR with MOLREP by adding each domain in > order. The first and second added domains, which are domain 1 from chain A > and domain 2 from chain B, are correct; however, when I tried to add the next > domain, R work/R free did not drop and clearly MOLREP did not find the > correct solution according to the density map. I also tried Phaser but the > result was the same. > > screen shot for domain 2 in chain A: https://ibb.co/YB2JnRR > > <a href="https://ibb.co/YB2JnRR";><img > src="https://i.ibb.co/0yq14DD/screenshot-on-wrong-domain.png"; > alt="screenshot-on-wrong-domain" border="0"></a><br /><a target='_blank' > href='https://imgbb.com/'>pio pio 3 menu</a><br /> > > In addition, I found an article on that data > (https://journals.iucr.org/d/issues/2008/01/00/ba5117/index.html) but I > failed to get the same result expect the first two domains. > > I wonder if anyone has done this tutorial before or has faced similar > problems in his own work. I have no idea on how to deal with it. Any > suggestions or comments will be helpful. Thanks in ahead. > > Link for that tutorial: > ftp://ftp.ccp4.ac.uk/tutorials/ccp4_phasing/7_domains/mth685.pdf > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
![https://i.ibb.co/0yq14DD/screenshot-on-wrong-domain.png"](https://i.ibb.co/0yq14DD/screenshot-on-wrong-domain.png"){kind=link}