the central blob looks too big for B or even for P or S.
Perhaps Zn, Cd? (although you didn’t add it, it might have been in the protein 
buffer or dragged through the purification by the protein?)

Mark J van Raaij
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
calle Darwin 3
E-28049 Madrid, Spain
Section Editor Acta Crystallographica F
https://journals.iucr.org/f/


> On 26 May 2021, at 15:08, leo john <ljohn16012...@gmail.com> wrote:
> 
> Hi Group
> Can you please suggest what this unmodeled blob can be (see appended picture)?
> I have Malonate, Boric Acid and Peg in my condition, and crystals were soaked 
> in GOL.
> 
> I have tried fitting PO4 and SO4 so far.
> 
> Thank You
> John
> <image.png>
> 
> 
> 
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