Hi Rob, There is no information provided to think what might be the problem with indexing. How confident are you in the direct beam coordinates? Are any of the cell lengths long-ish? It may not be enough but at a minimum, two images 90 degrees apart might be helpful. Preferably, they would be taken in the orientation with the best-looking diffraction and 90 degrees away from it. Also, if you could indicate presumed and refined direct beam positions on those images, it could be informative. Cheers, Nukri
On Mon, Jul 5, 2021 at 8:55 AM Robert S Phillips <p...@uga.edu> wrote: > I collected data last week on crystals of tyrosine phenol-lyase obtained > under new conditions. The data have higher resolution than previous > crystals, to 1.5 A. However, I can't get them to index in any space group > but P1. Usually, the space group is P21212. The self-rotation function is > attached. The P1 data will give a molecular replacement solution, but it > does not refine below 0.46. The P1 asymmetric unit fits a dimer, but the > assembly is a tetramer. In the map, I can see the difference peaks from > the other dimer of the tetramer. What could be causing this problem? > > Rob > > Robert S. Phillips > Professor of Chemistry and of Biochemistry and Molecular Biology > University of Georgia > Athens, GA 30602 > Phone: (706) 542-1996 > Fax: (706) 542-9454 > E-mail: rsphill...@chem.uga.edu > Web: http://tryptophan.net > <https://pod51004.outlook.com/owa/redir.aspx?C=ccbf42ffea5f48b1bf8e9bb950454bab&URL=http%3a%2f%2ftryptophan.net> > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
