Hi Jon, Thanks for the reply. I don't think this is acetylation, because I only see this density in the crystals that grew with malonate. In other conditions doesn't show anything like that. So I thought it'd be the malonate. But I'll check on that. Thanks for the suggestion.
Kind regards Ana On Mon, 16 Aug 2021 at 16:56, Jon Cooper <jon.b.coo...@protonmail.com> wrote: > Hello, could the His be partially acetylated? > > Best wishes Jon.C. > > > Sent from ProtonMail mobile > > > > -------- Original Message -------- > On 16 Aug 2021, 14:52, Ana Ebrecht < anaebre...@gmail.com> wrote: > > > Dear all, > > I am building the structure of a protein that was crystallized in 0.2 M > sodium malonate pH 5.0, 20% w/v polyethylene glycol 3,350. > During the refinement, we found what we think is a malonate molecule in > the active site, but it seems like is bound somehow to the histidine > (this His is the catalytic residue of the enzyme), almost like a covalent > interaction. Under other conditions of crystallization, the protein bound > a sulfate and an acetate in the site but did not show this type of > interaction with the histidine. > > We couldn't find anything that explains a reaction between the malonate > and the histidine. > Does anyone have experience with this reaction or have seen something > similar before? > > Thanks > Kind regards > Ana > > > > > > [image: malonate.jpg] > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
