Dear All,Sorry for disturbing you all with my current inquiry. However, I am in the process of designing and expressing soluble proteins and I thought to ask for your valuable advice. 1-What would be your first choice (protein-based) fluorophore to link it to the soluble protein at its C-terminus?2-Would this fluorophore be suitable for carrying later experiments in vivo and in vitro withstanding such conditions and still having detectable fluorescence signal (medium to high)?3-Would this fluorophore of choice stand denaturing and renaturing conditions, as refolding the protein chimera (soluble protein + the linked fluorophore) from bacterial inclusion bodies that were dissolved in 8M Urea or 6M Guanidine-HCl?4-Have you had success with producing APC or R-PE in such manner (secreting them from insect cells or refolding them from bacterial inclusion bodies)? Thank you in advance.Best regards,Samer
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