Dear Dhiraj, It may be perceived as “slow”, but we (still) use the Bac-to-Bac system from ThermoFisher (previously Invitrogen). This coupled with the pFastBac, MultiBac or biGBac vector systems generally works well; some proteins/complexes may express better in High 5 cells over Sf9/Sf21.
With regards, Antony. Antony W Oliver FHEA, PhD Faculty Senior Research Fellow Genome Damage and Stability Centre Science Park Road University of Sussex Falmer, Brighton, BN1 9RQ (office): +44 (0)1273 678349 (lab): +44 (0)1273 677512 antony.oli...@sussex.ac.uk<mailto:antony.oli...@sussex.ac.uk>; (he/him) https://www.sussex.ac.uk/lifesci/oliverlab https://tinyurl.com/aw-oliver https://orcid.org/0000-0002-2912-8273 http://www.sussex.ac.uk/lifesci/internal/staff/support From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of "Srivastava, Dhiraj" <dhiraj-srivast...@uiowa.edu> Reply to: "Srivastava, Dhiraj" <dhiraj-srivast...@uiowa.edu> Date: Monday, 7 February 2022 at 16:24 To: "CCP4BB@JISCMAIL.AC.UK" <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Baculovirus expression system Hi All sorry for the question not related to crystallography. What is the baculovirus expression system that people use these days to get good yield in less time? Thank you Dhiraj ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
