Dear all
The next call for beamtime during the period October 2022 – March 2023 at Diamond MX beamlines I03, I04, I04-1, I23 and I24 is now open with a deadline of 17:00 GMT on 30th March 2022. We provide a broad range of facilities to the UK and international community with very flexible and responsive access – see https://www.diamond.ac.uk/Instruments/Mx.html for details. Remote, automated and on-site access is available to fast, tunable and microfocus beamlines (some equipped with multi-axis goniometry) alongside specialisms in serial synchrotron crystallography (pump-probe, fixed targets, viscous extruders and more at I24;<https://www.diamond.ac.uk/Instruments/Mx/I24/I24-serial.html> contact Robin Owen), long wavelengths for phasing and metal identification (I23<https://www.diamond.ac.uk/Instruments/Mx/I23.html> – see below for more details) and fragment screening at XChem-I04-1<https://www.diamond.ac.uk/Instruments/Mx/Fragment-Screening.html>. Data are analysed automatically and downstream pipelines for difference map calculations, molecular replacement and experimental phasing run as required. We run AlphaFold2 on sequences submitted prior to data collection for use in our pipelines. Results are made available via our ISPyB/SynchWeb webpages. Access for non-proprietary use is free at the point of access with Dewar shipping covered for UK and European users and accommodation and subsistence support for on-site visits. More information on additional access and support for European-based research groups, which is currently supported by iNEXT-Discovery and INSTRUCT-ERIC, can be found here https://www.diamond.ac.uk/Users/Support-for-European-Access-to-Life-Sciences.html. Access is normally via the Block Allocation Group (BAG)<https://www.diamond.ac.uk/Users/Apply-for-Beamtime/BAG-Access.html> system for a period of 2 years or through standard access<https://www.diamond.ac.uk/Users/Apply-for-Beamtime/Standard-Access.html> for single experiments. To apply please follow the instructions at https://www.diamond.ac.uk/Users/Apply-for-Beamtime.html and complete your application by 17:00 GMT on 30th March. In addition to the current call, in situ crystallisation plate room temperature data collection at VMXi<https://www.diamond.ac.uk/Instruments/Mx/VMXi.html> is available through direct contact with the beamline team (further information below). Similarly, the micro/nanofocus beamline VMXm<https://www.diamond.ac.uk/Instruments/Mx/VMXm.html> will open a commissioning call from April. Best wishes Dave Hall on behalf of the MX Group at Diamond Additional information * Unattended data collection Remote access to MX beamlines has been routine at Diamond for many years. At the start of the pandemic we introduced an automated unattended data collection (UDC) pipeline<https://www.diamond.ac.uk/Instruments/Mx/I03/I03-Manual/Unattended-Data-Collections.html> to several of our beamlines. Since operation started mid 2020 we have collected over 60,000 data sets including many COVID-19 targets of interest. Typically we turn around high quality data sets within 1-4 days from arrival at Diamond via this access route. * The benefits of long wavelength data collection AlphaFold2 and RosettaFold are now able to provide good MR models. However, anomalous scattering can still be important for some borderline cases. In case you have a project which requires phasing, check our S-SAD Phasing App<https://www.diamond.ac.uk/Instruments/Mx/I23/resolution-requirement-phasing-app.html>. Identification of lighter atoms can be routinely done on I23. The beamline is unique and can access the absorption edges of Ca, K, Cl, S and P. Several successful studies have been published, e.g. High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter cresentus cells.<https://www.sciencedirect.com/science/article/pii/S0969212621003804?via%3Dihub> A dedicated rapid access route for European groups is available through iNEXT discovery for I23. For further information see the I23 webpage and contact Armin Wagner (armin.wag...@diamond.ac.uk<mailto:armin.wag...@diamond.ac.uk>) * In situ room temperature data collection (in crystallisation plates) at VMXi. Users provide either protein sample which we use to grow crystals, or bring suitable crystallisation plates. Plates are imaged locally and users remotely select crystals (point and click in ISPyB) and specify appropriate data collection parameters. Data collection is fully automated and datasets from single or multiple crystals accessed via ISPyB. Complete room temperature datasets can often be produced from a handful of crystals as small as 10-20 microns. -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. 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