Problematic is low multiplicity, not high, I’d say... Best
Rafael Marques da Silva Mestre em Física Biomolecular Universidade de São Paulo Bacharel em Ciências Biológicas Universidade Federal de São Carlos phone: +55 16 99766-0021 "A sorte acompanha uma mente bem treinada" ________________________________________________ De: Winter, Graeme (DLSLtd,RAL,LSCI)<mailto:00006a19cead4548-dmarc-requ...@jiscmail.ac.uk> Enviado:segunda-feira, 19 de setembro de 2022 16:27 Para: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Assunto: Re: [ccp4bb] Multiplicity is more than 20 Hi Prasun You just measured the reflections a lot. 33 is fine. More likely even with cubic space groups I’d love to know where you got the idea of the limit from. I have data sets with more than 100 fold multiplicity The R conversation I’ll leave to others Best wishes Graeme On 19 Sep 2022, at 20:32, Prasun Kumar <prasun.ku...@bristol.ac.uk> wrote: Hi All: I have collected a dataset for a crystal of a 30 residues long helical peptide that makes a trimer in the solution. I also solved the structure to get a trimer. My issues start when I start preparing for a deposition. Details about the data: space group: I 21 3 Resolution: 1.6 Current Rfree/ Rwork: 0.21/0.19 Problems: According to Aimless, Multiplicity: 33.9, and I understand that the value should be less than or equal to 20. Does it mean that I have a lot of random noise or ice rings or something similar? For the inner shell, R work is also higher than R free. Please guide me in solving the above issue. Thank You Prasun To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 -- This e-mail and any attachments may contain confidential, copyright and or privileged material, and are for the use of the intended addressee only. If you are not the intended addressee or an authorised recipient of the addressee please notify us of receipt by returning the e-mail and do not use, copy, retain, distribute or disclose the information in or attached to the e-mail. Any opinions expressed within this e-mail are those of the individual and not necessarily of Diamond Light Source Ltd. Diamond Light Source Ltd. cannot guarantee that this e-mail or any attachments are free from viruses and we cannot accept liability for any damage which you may sustain as a result of software viruses which may be transmitted in or with the message. Diamond Light Source Limited (company no. 4375679). Registered in England and Wales with its registered office at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/