H Everyone, Please join us the upcoming Cancer therapeutics webinar with Dr. Basil Greber on Oct 18:
Dr. Basil Greber from the Institute of Cancer Research in London, UK, will demonstrate how the Glacios 2 Cryo-TEM facilitated the discovery of next-generation therapeutics. Dr. Greber and his team used the Glacios 2 Cryo-TEM in their efforts to make CDK-activating kinase (CAK) amenable to structure-based drug design. First, they determined the structures of the human CAK bound to nucleotide analogues and two inhibitors. They then used the Glacios 2 Cryo-TEM equipped with a Thermo Scientific Selectris X Imaging Filter and a Thermo Scientific Falcon 4i Direct Electron Detector to uncover the structural basis of inhibitor selectivity, which required rapid acquisition of high-quality datasets for large numbers of different inhibitors. This workflow enabled 3D reconstruction of the CAK at 3.5 to 4.5 Å resolution within one hour from the start of data collection and at approximately 3 Å resolution within four hours. Finally, they obtained more than a dozen high-resolution structures of the 85 kDa CDK-cyclin-module of the human CAK bound to inhibitors and nucleotides at up to 1.8 Å resolution using a Thermo Scientific Krios G4 Cryo-TEM with a cold-FEG. These structures provided detailed insight into inhibitor binding and suggested a mechanism for target selectivity that they are currently testing. Register here!<https://www.thermofisher.com/us/en/home/about-us/events/industrial/ls-cryo-electron-microscopy.html> Best regards, Jeff ____________________________________________________________________ Jeffrey Lengyel, Ph.D. Director, Product Marketing, Cryo-EM, Electron Microscopy Life Sciences Materials & Structural Analysis Thermo Fisher Scientific 5350 NE Dawson Creek Drive | Hillsboro, OR 97124 Phone 503 560 9184 jeffrey.leng...@thermofisher.com<mailto:jeffrey.leng...@thermofisher.com> [Text Description automatically generated]<https://www.thermofisher.com/us/en/home/electron-microscopy/products/transmission-electron-microscopes/glacios-cryo-tem.html> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
