Hi Zeya, Did you cryoprotect with glycerol perhaps?
Dave ------------------------------- Prof. David M. Lawson Department of Biochemistry and Metabolism, John Innes Centre, Norwich, NR4 7UH, UK. Tel: +44-(0)1603-450725 Web: https://www.jic.ac.uk/people/david-lawson Email: david.law...@jic.ac.uk<mailto:david.law...@jic.ac.uk> From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of zeyaul islam Sent: 21 February 2023 11:13 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Unidentified density You don't often get email from zeya1...@gmail.com<mailto:zeya1...@gmail.com>. Learn why this is important<https://aka.ms/LearnAboutSenderIdentification> Dear all We have solved the structure of nanobody at 1.25 angstrom. We observe some unidentified density near the serine. Please have a look at the figures. Protein was purified in PBS buffer (137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4) and the crystallisation condition has 0.1 M BIS-TRIS pH 5.5, 2.0 M ammonium sulfate. Any ideas what this could be? I tried SO4 and PO4 but didn’t satisfy the observed electron density. I appreciate any help you can provide. Thanks Zeya Zeyaul Islam, PhD QBRI - Qatar Biomedical Research Institute P.O. Box: 34110 Doha – Qatar Tel: +974 445 46690 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
