Going back to RIP phasing methods :-) So Harry in your particular case occupancy of zero would actually reflect reality for those “combusted” atoms.
Jürgen > On Mar 10, 2023, at 11:56 AM, Harry Powell > <0000193323b1e616-dmarc-requ...@jiscmail.ac.uk> wrote: > > Hi folks > > One other thing that I haven’t noticed anyone mentioning yet (sorry to those > who have mentioned it!!) is that you may not see your sidechain atoms in > density because they are not there at all, in spite of what you may have had > in the original protein, or even if the atoms were really there in the > crystal _before_ exposure to the beam. > > The coordinates are supposed to be what you actually find, not what you hope > is there. > > Just my two ha’porth > > Harry > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing > list hosted by www.jiscmail.ac.uk, terms & conditions are available at > https://www.jiscmail.ac.uk/policyandsecurity/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
