Hi,
As the others have already mentioned, it is hard to get any decent MR solution
at this resolution. The best thing to do would be to set up more crystal
plates and harvest crystals growing at different conditions. You also have not
mentioned where you diffracted your crystals. If you did it using an in-house
system you should consider a synchrotron light source.
Best wishes
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
"A sorte acompanha uma mente bem treinada"
________________________________________________
De: Paul Emsley<mailto:pems...@mrc-lmb.cam.ac.uk>
Enviado:terça-feira, 21 de novembro de 2023 12:08
Para: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
Assunto: Re: [ccp4bb] low resolution data refinement
On 21/11/2023 12:02, Yahui Liu wrote:
>
> Dear all,
> I got a protein crystal dataset of 4.3 A and would like to some the
> structure with MR.
> Now I am suffering with the refinement.
Just for the record, then intention of refinement is not to make you suffer.
Paul.
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