This should all go fine. You can maxi- or mini-prep the plasmid DNA from the expression strain and transform it back into a cloning strain for sequencing, etc.
Best wishes, Jon Cooper. [email protected] Sent from Proton Mail mobile -------- Original Message -------- On 19 Feb 2024, 00:49, Javier Gonzalez wrote: > Dear all, > I'm sure this issue comes up very often, but for the first time in our lab we > need to recover a pET-type expression plasmid from a BL21-like E. coli strain > (NEB's T7 Express). > I know a RecA+ strain is not suitable for plasmid production, but the basic > plan is to grow and mini-prep the cells to recover the plasmid and later > transform another E coli strain (Top10) to make frozen stocks and for plasmid > production. > Is this a regular practice or is there any known protocol we should follow? > Any advice will be greatly appreciated. > > Best wishes, > Javier > > -- > > Dr. Javier M. González > Instituto de Bionanotecnología del NOA (INBIONATEC-CONICET) > Universidad Nacional de Santiago del Estero (UNSE) > RN9, Km 1125. Villa El Zanjón. (G4206XCP) > Santiago del Estero. Argentina > > Tel: +54-(0385)-4238352 > [Email](mailto:[email protected]) [Twitter](https://twitter.com/_biojmg) > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
