I believe someone has already mentioned matrix seeding- taking the crystals you currently have, making microseeds and then using this in a new screen (whatever your favourite initial screens may be). This can give you different conditions that will give you better crystals as you already have seeds to nucleate from. Best of luck, tom ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of 白雪慧 <zb20193020...@cau.edu.cn> Sent: Friday, May 31, 2024 12:32 PM To: CCP4BB@JISCMAIL.AC.UK <CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Crystal optimization
You don't often get email from zb20193020...@cau.edu.cn. Learn why this is important<https://aka.ms/LearnAboutSenderIdentification> Thank you very much for your suggestions. I have a question. My crystal grows microcrystals under multiple conditions, as shown in the figure. After orthogonal optimization of the precipitant and pH, the crystal growth is still very small and difficult to obtain diffraction. What is the method to optimize and increase the crystal size in this situation? ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
