Dear Reza,
the stability of these complexes is variable and depends on many factors
including your protein, the pH of the buffer etc. I would recommend
aluminium fluoride as the most stable (below pH8) as it is present in
solution and has a the highest charge to size ratio so binds tightly in
the active site. However, if your goal is to study the ground state use
BeF3-. We have a preprint out that goes into much more detail - see
https://www.biorxiv.org/content/10.1101/2024.03.25.586559v1.full
Best wishes, Matt
On 03/07/2024 16:58, Reza Khayat wrote:
Hi,
Sorry for the non crystallography question. We need to make some ATP
analogues (ADP BeFx, ADP MgF3, and ADP AlFx). I have attained
protocols from manuscripts for making these; however, am concerned
that something important in the protocol may be lacking. Also, how
stable are these samples? Thanks.
Best wishes,
Reza
Reza Khayat, PhD
City College of New York
Associate Professor
Department of Chemistry and Biochemistry
Co-Director NIH G-RISE program
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