This may not necessarily be genetics.  Some years ago a fellow UK Show/Breeder had - 
you guessed it a pick of litter bitch, which was vaccinated at around 12 weeks of age. 
The dog became poorly but quickly recovered (maybe a 24 hr thing) however one eye went 
blue!  The other remained dark liquid brown.

They never did get a definitive answer on the subject, however they were convinced 
that the vaccination was the cause of this anomaly.

Regards
Maryann Hogan
Stavonga Cavaliers (UK)

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