Scientists Tout Method of Attacking Tumors 

By DIEDTRA HENDERSON, AP Science Writer 

WASHINGTON - Texas researchers say they have perfected a method to 
deliver cancer treatment directly into tumors, bypassing healthy 
tissue. 

The study was done on mice, but human trials could begin soon, said 
Dr. Michael Andreeff, one author of the study in Wednesday's issue 
of the Journal of the National Cancer Institute (news - web sites). 


The research team used the benefits of a known anticancer therapy, 
interferon beta, that can kill cancer cells. In practice, that 
therapy has proven problematic. It causes toxic side effects and its 
benefits disappear within minutes of patients getting their shots. 


The research team worked around those problems by manipulating a 
certain type of stem cells to encode the interferon beta gene. The 
stem cells then move like guided missiles, targeting tumor cells and 
producing high concentrations of therapeutic proteins within the 
tumor cells, Andreeff said. 


Besides taming toxic side effects, the cancer treatment stuck around 
in the tumor longer, he said in an interview. 


Mice with human breast cancer treated with the engineered human stem 
cells survived for 60 days, according to the JNCI paper. Mice 
treated with interferon beta alone lived for 41 days. Untreated mice 
survived for 37 days. Meanwhile, mice with melanoma treated with the 
stem cells survived 73.5 days, compared with 30 days for untreated 
mice. 


Andreeff said he's working on a protocol for a clinical trial to 
test the procedure in humans within a year, if the Food and Drug 
Administration (news - web sites) agrees. Patients would be infused 
with the stem-cell-delivered anticancer treatment four times a week, 
said Andreeff, a professor in the departments of blood and marrow 
transplantation and leukemia at the University of Texas M.D. 
Anderson Cancer Center. 


The targeted delivery of anticancer therapy to tumors builds on what 
researchers already know about how wounds heal. 


The specialized stem cells � known as mesenchymal stem cells � come 
from bone marrow and help maintain healthy connective tissues. When 
new tissue is needed to heal wounds or form scars, those special 
stem cells swell in number. 


Even though they're tumors, the malignant cells act just like "never-
healing wounds," Andreeff said. Half the tumor is made up of stomal 
cells that provide structural support. For the body, forming that 
tumor support structure is much like healing wounds and forming 
scars. 


Enter the specialized stem cells. Giving them the clues they need to 
take on the construction duties of stomal cells delivers the cancer-
busting ability directly to tumors. 


Andreeff's research team did not see engineered stem cells drift 
into healthy organs like the lungs, liver, spleen, kidney or 
muscles. 


But because the stem cells are driven by a duty to help, that means 
a wound elsewhere in the body could distract some from reaching 
tumors. 


"Any wound that's active, that requires repair, would be a target," 
Andreeff acknowledged. That means doctors would need to screen 
patients carefully to ensure the therapy is not attempted on people 
who had undergone recent surgery, for example. 


The work builds on the promise of using stem cells to hunt down 
brain tumors, outlined in a 2000 paper in the Proceedings of the 
National Academy of Science. 


"It was my hope that other investigators looking at other areas, 
using other stem cells ... would seize upon this," said Dr. Evan 
Snyder, director of the Burnham Institute's stem cell program in La 
Jolla, Calif., and an author of the earlier paper. Snyder called the 
JNCI article "important, because it's proof of concept.... In this 
case, a mouse is simply a surrogate for a human being." 


___ 

   



On the Net: 

Journal of the National Cancer Institute: 
http://jncicancerspectrum.oupjournals.org/ 








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