On 26/03/12 19:58, Alok Shenoy wrote:
Hello,
Problem:
1) Chain A has a couple of residues missing. The numbering shows 231 232 234.
Clearly marking out the spot where the residue is missing. Sequence shows that
there is an aspartic acid missing in position 233. Problem is - both 232 and
234 are connected quite normally,
AFAICS, this is not a problem - this is what should happen (if I have
understood the problem correctly). If there is a gap, you need to use a
GAP card.
inserting a terminal residue at 232 (alanine at first) results in absolutely
weird connections.
Yes, initially, it may well do. After regularization, things are tidied
up - for me at least.
2) At some points, there is no backbone -by which I mean - lets say residue 100
and 101 side chains are showing up (and have appropriate electron density).
What is missing is the peptide bond that connects these two residues (again -
electron density for the backbone is present). Is there a way to fix this?
So are you saying that the backbone atoms are in the file but are not
displayed...?
or perhaps
The backbone atoms are not in the file and you want to build them?
Can someone walk me through the solution to this problems?
If you A and B are NCS-related then the Copy NCS Residue Range function
might be useful.
Paul.
