Hi everyone, I would be very grateful if you could help me with the following:
I am refining a protein that has been treated with chymotrypsin prior to crystallisation. As a results, there are nicks in the chain but the chain has remained associated due to SS bonds and Ca sites. How could I model these breaks in Coot? The program tends to reform the peptide bond whatever I do to keep it apart... I would like to keep the same identifier for the chain and to be able not to form bonds when required. Many thanks for your help. With regards, Chrislaine.
