Hello, is flipping a His side chain using the button on the right-hand toolbar working for you as expected? In our hands, starting with version 0.8.9 (?), the "side chain flip", applies a 180 degree chi-2 rotation keeping the imidazole moiety fixed and disrupting the protein main chain. Gln and Asn flips work as expected. Thanks. Wolfram
- [COOT] Flip (histidine) sidechain 180 degrees wtempel