On 15/07/2019 10:22, Paul Emsley wrote: > On 15/07/2019 02:48, Ahmad Khalifa wrote: >> I know I can either force beta strands or alpha helix restraints >> while refining/regularizing, but what about 3-10 helices?! >> > > For a 3-10 helix, you will have to add the bond restraints one by one: > > Extensions -> Modules -> User Defined Restraints -> Restraints -> Add > Distance Restraint > > or use ProSMART to generate restraints over your atom selection - they > can then be imported into Coot using "Read Refmac Extra Restraints" >
For a bit of amusement, here is an example I made a little while ago illustrating how Coot regularizes/minimizes 3-10 helicies with its own "force-field" (not fitting to density): https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/movies/3-10-helix-regularize.mov Coot is often "on your side" when it comes to rebuilding - but not in this case... you need extra help. Paul. ######################################################################## To unsubscribe from the COOT list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=COOT&A=1
