On 20/09/2020 17:29, Qiuye Li wrote:
Thanks for the suggestion, it looks map segmentation and coot do not
work well with each other, see the tests I did below:
1. .mrc map directly from relion:
in Chimera: OK
in Coot: OK
2. segmented map generated from Chimera:
what do you mean by "segmented"?
in Chimera: OK
in Coot: displayed as replicates
When I segment the map, the box dimensions and map threshold level
were changed, would these cause problems?
I am not really sure what you mean by the map threshold level. It's
something of a surprise that the box (cell) dimensions have changed.
Maybe that's a result of reboxing? But I doubt that that would cause
problems to Coot.
It looks like the map is interspersed with sections that have zero
value. However, from the orientation axes top left it doesn't seem that
the sections lie along the direction of a cell axis.
Paul.
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