On 13/10/2020 15:40, Sorbhi Rathore wrote:
— The RNA segment that I am trying to fit is ~130 bp.
That should be fine. A bit slow maybe.
— If I try to fit it without restraints, the RNA helix doesn't go into the density.
Maybe try fitting to a blurred map?
When I drag or remove the restraints it collapses (base pairing, planarity is destroyed.)
(i) Too fast or too extensive drag, try 10 micro-yanks instead of one big yank
(ii) alpha too high (iii) map to model weight too high But that will be a thing of the past in 0.9.1. Paul. ######################################################################## To unsubscribe from the COOT list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=COOT&A=1 This message was issued to members of www.jiscmail.ac.uk/COOT, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
