Dear all, I have what feels like a trivial question about modelling in coot, but unfortunately I have not managed to solve it myself.
I would like to create a bond between a P-tRNA and nascent chain and then subsequently use RSR in Coot for modelling. What I gathered so far is that I should use acedrg to create the restraints of the linked unit (in my case "A" to "VAL"). Therefore, I first followed the tutorial in the Coot development blog which worked well. But when I try to do the "real world" example with my model, acedrg crashes (and I could not find a useful error message). What I could figure out is that it has something to do with the RNA-nucleotide being referred to as "A", because linking an imported "AMP" molecule to valine as well as my whole nascent chain works as it should. Therefore, I wanted to ask if you could briefly tell me what is the correct way to achieve this type of linkage in Coot? Many thanks for reading and best wishes, Helge P. S. I have modified Oli Clarke's trimmings to automatically load the "Restraints" menu with "add_module_restraints()", but this is only partially working as the menu is not showing all entries upon starting coot. I have to still manually load "Calculate -> Modules -> Restraints" to get the rest of the entries to show. Is there something I could do to have the full menu from the get go? ######################################################################## To unsubscribe from the COOT list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=COOT&A=1 This message was issued to members of www.jiscmail.ac.uk/COOT, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
