Hi, I am currently working on refining a protein/RNA complex. I am using coot to build my model and am wondering if anyone has any experience in dealing with RNA's modifications on them? I am working with a N6-methyl-adenine and am having problems with it in coot. In the library, there is a 6MZ.I have tried the script: mutate-by-overlap imol chain-id residue-no tlc (mutate-by-overlap imol chain-C residue-1 6MZ), but it did not work. I also tried to delete “A” and add “6MZ”, but I can not link “6MZ” with other nucleotides. How do I add restraints so I can regularize or real space refine? What can I do? Thank you, Zoey
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