On 31/12/2023 16:01, Gang Du wrote:

Hi Coot,
Hi Gang  Du.

I solve the structure from cryosparc.  The resolution is 3.2A. But I have a problem opening the map by coot. The chimera and ChimerX can open the map successfully and correctly. You can see the following image. But it cannot be opened successfully and correctly by coot. It looks like there are some problems.  You can also see the following image. Do you know the reason?


Cute structure (well, reconstruction at the moment).

What you are seeing is (merely) Coot's rendering of the map. It is not unusual to be confused by what Coot is showing you at this stage.

What is the radius of the map in ChimeraX? Set the same map radius in your Coot and you should see the same map (notice that you have the contour level a lot lower in Coot) - rendered in Coot style of course.

Also, I notice that you have green and red levels, which suggests that you have opened this as a difference map. I would not recommend that.

i.e. you are looking in the wrong place in the wrong style at the wrong contour level. Hopefully all of that should be easy to fix and you can get on with adding some atoms - exciting!

Regards,

Paul.


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