-Caveat Lector-

"Using reverse-transcription PCR (polymerase chain reaction), the scientists
identify the virus as a West Nile virus (WNV) virtually identical to a West
Nile virus that was found in an outbreak in Israel in 1998. It is also very
similar to a virus found in Egypt, they report. ... These similarities
suggest a Middle Eastern origin for the New York outbreak."
==================================================================

http://unisci.com/stories/19994/1203994.htm

Complete Genome Of West Nile Virus Identified

Dr. Ian Lipkin of the University of California at Irvine and his colleagues
have identified 100 percent of the genome of the West Nile virus that caused
the encephalitis outbreak in New York in August of this year.

The report appears in this week's Lancet.

This study marks the first time any scientist has cloned a viral genome in
the human brain, using a technique which may help identify causes of
encephalitis in other outbreaks.

Using reverse-transcription PCR (polymerase chain reaction), the scientists
identify the virus as a West Nile virus (WNV) virtually identical to a West
Nile virus that was found in an outbreak in Israel in 1998. It is also very
similar to a virus found in Egypt, they report.

These similarities suggest a Middle Eastern origin for the New York
outbreak.

The West Nile virus is among the Flaviviridae, which also include human
pathogens such as yellow fever, dengue, Japanese encephalitis and St. Louis
encephalitis. Normally, WNV infections are mild, but they can result in
encephalitis and in death.

WNV is transmitted by mosquitoes, but birds can carry it. The virus is now
known to have been responsible for bird deaths in New York, New Jersey and
Connecticut. The Lancet article notes that WNV infection was not recognized
in the Americas until August of this year, although there were reports of
WNV epidemics in Israel, France, South Africa and Romania.
Noting the existence of two international airports in the New York City area
where WNV was prevalent, the article suggests that potential routes for
introduction of the virus into the Eastern United States include importation
of infected birds, mosquitoes and viremic human beings.

Co-authors include Lipkin, Xi-Yu Jie, Thomas Brieze, Ingo Jordan, Andrew
Rambadt, Hen Chang Chi, John S. Mackenzie, Roy A. Hall and Jacqui Scharret.


[Contact: Andrew M. Porterfield <mailto:[EMAIL PROTECTED]>]

03-Dec-1999


=======================
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