Same for Ubuntu 16.04.4 and nautilus 3.14.3:

$ LC_ALL=C nautilus

(nautilus:2903): GLib-GIO-CRITICAL **:
g_dbus_interface_skeleton_unexport: assertion
'interface_->priv->connections != NULL' failed

(nautilus:2903): GLib-GIO-CRITICAL **: g_dbus_interface_skeleton_unexport: 
assertion 'interface_->priv->connections != NULL' failed
Could not register the application: Timeout was reached

(nautilus:2903): Gtk-CRITICAL **: gtk_icon_theme_get_for_screen:
assertion 'GDK_IS_SCREEN (screen)' failed

(nautilus:2903): GLib-GObject-WARNING **: invalid (NULL) pointer
instance

(nautilus:2903): GLib-GObject-CRITICAL **: g_signal_connect_object:
assertion 'G_TYPE_CHECK_INSTANCE (instance)' failed

$ lsb_release -a
LSB Version:    
core-9.20160110ubuntu0.2-amd64:core-9.20160110ubuntu0.2-noarch:security-9.20160110ubuntu0.2-amd64:security-9.20160110ubuntu0.2-noarch
Distributor ID: Ubuntu
Description:    Ubuntu 16.04.4 LTS
Release:        16.04
Codename:       xenial

$ nautilus --version
GNOME nautilus 3.14.3


Workaround:
$ killall nautilus

-- 
You received this bug notification because you are a member of Desktop
Packages, which is subscribed to nautilus in Ubuntu.
https://bugs.launchpad.net/bugs/1290310

Title:
  nautilus does not start

Status in nautilus package in Ubuntu:
  Incomplete

Bug description:
  I tried to launch nautilus but it did not start so i tried from alt+f2
  and terminal but it did not start I got following message


  j0053@j0053-hp-probook-4420s:~$ nautilus
  Could not register the application: Timeout was reached

  ProblemType: Bug
  DistroRelease: Ubuntu 14.04
  Package: nautilus 1:3.10.1-0ubuntu7
  ProcVersionSignature: Ubuntu 3.13.0-16.36-generic 3.13.5
  Uname: Linux 3.13.0-16-generic i686
  NonfreeKernelModules: wl
  ApportVersion: 2.13.3-0ubuntu1
  Architecture: i386
  CurrentDesktop: Unity
  Date: Mon Mar 10 14:42:18 2014
  GsettingsChanges:
   b'org.gnome.nautilus.list-view' b'default-visible-columns' b"['name', 
'size', 'type', 'date_modified', 'where']"
   b'org.gnome.nautilus.list-view' b'default-column-order' b"['name', 'size', 
'type', 'date_modified', 'group', 'where', 'mime_type', 'owner', 'permissions']"
  InstallationDate: Installed on 2012-03-24 (715 days ago)
  InstallationMedia: Ubuntu 12.04 LTS "Precise Pangolin" - Alpha i386 (20120324)
  SourcePackage: nautilus
  UpgradeStatus: Upgraded to trusty on 2014-01-30 (39 days ago)

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