If this is a new environment with existing source code, did you install all 
the requirements?  eg, is there a requirements.txt file in the project 
root, and did you execute pip install -r requirements.txt when the virtual 
env was activated?

If so, try running pip install python-dotenv

Your error message says it can't find that module, so I suspect the 
python-dotenv package hasn't been installed to your virtualenv

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