Hi ECOLOG, I am a graduate student attending California State University, Fullerton and am currently working on a landscape genetics study that involves the use of microsatellite markers to determine patters of gene flow and population structure in northern grasshopper mice. I am in need of some advice in order to choose an appropriate method for scoring alleles. While the current literature seems to favor sequencing( I do too),I doubt my budget can support an 300 sample sequencing enterprise. I have read that comparing silver stained polyacrylamide gels to a size standard also works. As does autoradiography. I am interested in hearing about the experiences folks have had with these older methods and if you could suggest ways and/or companies of making sequencing more affordable.
Please feel free to email me directly at kflores2...@csu.fullerton.edu Thanks, Karla
