Ecologgers, My lab has been studying the barnacle Pollicipes elegans, which has an anti-tropical distribution across the Eastern Tropical Pacific. Populations are found in northern Mexico, El Salvador and Peru/Ecuador and the barnacle is absent between these locations. We are interested in examining the role of thermal tolerance in setting the range limits of this species and potentially local adaptation among populations. My advisor primary works on larvae and we have already run our larval experiments. I decided to also run some experiments on my own with adults incorporating some biochemical assays, which is somewhat outside my lab's expertise. I am looking for citrate synthase and HSP 70 assay kits that have been used with other marine crustaceans. Ideally the kits would be all inclusive and include most, if not all, of the reagents needed for the assays. Any manuscripts where purchasable kits were used or suggestions of kits others have used in the past would be greatly appreciated.
Cheers, Sam Crickenberger
