Hi everyone, I have a graduate student who is trying to study how the abundance of several antibiotic resistance genes changes with urbanization along a river flow path. Unfortunately, her progress has been stymied for several months due to a problem getting orders filled through ATCC. It's a huge fiasco, and the upshot is that she cannot get access to the standards she needs for some of her qPCR assays.
I am writing in search of a few generous and sympathetic colleagues who happen to have some resistance genes cloned in plasmid/culture they would be willing to share (must be BSL1). Her preferred target genes are listed below, but this is very flexible depending on what is available. mcr1, ermB, strA, qnrA, sul1, sul2, tetB We can cover shipping costs and possibly arrange for modest compensation. More importantly, you would earn a huge shout-out in the "Acknowledgments" section of her M.S. thesis and the eternal gratitude of a struggling young scientist whose graduation keeps getting delayed by order processing and shipping problems far beyond her control. We would also appreciate suggestions of alternative vendors, though we have contacted the major ones to no avail (DSMZ, Addgene, BEI, and JCM). Please direct questions and responses to me directly to me at rbfrank...@vcu.edu or rima.frank...@gmail.com Thanks! Rima Rima Franklin Associate Professor Interim Associate Chair VCU Department of Biology www.people.vcu.edu/~rbfranklin 804-828-6753 (office) / 804-828-0125 (lab) rbfrank...@vcu.edu
