Thank you very much for your reply. The very low colonization rates for
one of the treatments are observed in all four blocks. I guess therefore
that I can exlude this treatment and test the relative effects of the
three remaining treatments in a 3-by-4 ANOVA followed by a multiple
comparison test.

L�naick  

Donald Burrill a �crit :
> 
> You really don't want to include a "zero mean zero variance" treatment
> in your analysis:  it will generate a main effect of treatment, and the
> "zero variance" part will cause your error mean square to be
> underestimated, leading to what one might call spurious sensitivity to
> treatment effects.  And it isn't as if you needed to find out from the
> analysis that this treatment differs from the others -- you know that
> already.
> 
> For the treatment that was "poorly colonized", is that true of all four
> blocks?  If so, I'd recommend eliminating that treatment from the
> analysis.  You have essentially no useful information about it, in terms
> of the variable you're analyzing.  (Except, perhaps, that the treatment
> is lethal.)  If the treatment really does have, as you write, zero mean
> and zero variance, you don't need a formal ANOVA to tell you that it
> differs from the other treatments.  In a 3-by-4 ANOVA you can then
> assess the relative effects of the other treatments.
> 
> If, instead, the "zero mean zero variance" phenomenon applies only to
> one of the 16 block-by-treatment conditions in your experiment, there is
> a way of adjusting for that particular condition while pursuing effects
> in the rest of the experimental design.  But this is not a standard form
> of analysis, and will take some explaining.  I'll wait for clarification
> before trying to describe it.
> 
> On Thu, 15 Apr 2004, Lenaick MENOT wrote:
> 
> > I have conducted a colonization experiment invloving four treatments
> > each with four replicates, the replicates were randomly placed in four
> > blocks. I would like to use a two way ANOVA without replication to
> > test the effects of treatments but one of the treatment was poorly
> > colonized and most of the taxa are lacking.
> >
> > My question is could I perform an ANOVA including the treatment with
> > zero mean, zero variance, should I exclude the treatment with zero
> > mean zero variance from the analysis or is there any special procedure
> > to deal with zero mean zero variance in an analysis of variance?
> >
> > Thank you for your help.
> >
> > L�naick Menot
> 
>  ------------------------------------------------------------
>  Donald F. Burrill                              [EMAIL PROTECTED]
>  56 Sebbins Pond Drive, Bedford, NH 03110      (603) 626-0816
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