Hi Peter Thanks for that.
Is it possible to preserve the fastq format? My input was fastq, I also put .fastq as my output, but it only gave me straight fasta Thanks Mick -----Original Message----- From: Peter Rice [mailto:p...@ebi.ac.uk] Sent: 01 October 2009 13:10 To: michael watson (IAH-C) Cc: 'emb...@emboss.open-bio.org' Subject: Re: [EMBOSS] Trim polyA from fastq files On 01/10/09 12:33, michael watson (IAH-C) wrote: > Hello > > I have some Illumina reads which have varying lengths of polyAs at the ends. > I want to trim these off. The data is in fastq format. > > Is this possible in EMBOSS? I have 6.0.1 and I tried trimest but it couldn't > read my fastq file Yes, but you need EMBOSS 6.1.0 to support FASTQ input. There are also a couple of patches that are needed for some FASTQ files. regards, Peter Rice _______________________________________________ EMBOSS mailing list EMBOSS@lists.open-bio.org http://lists.open-bio.org/mailman/listinfo/emboss