I was able to reproduce this issue with primer3-2.3.6 as well. On Sa, 2015-03-28 at 22:42 +0100, Horea Christian wrote:
> As seen in this discussion [https://www.biostars.org/p/102268/] > primer3 (primer3_core) needs the SEQUENCE_TEMPLATE to be set in a > config file. Apparently eprimer does not do this. > > This discussion > [https://www.mail-archive.com/[email protected]/msg01980.html] > is referenced in the above thread to support the claim that primer32 > should be used instead, but it actually states that the proper > function to use for primer3-2.3.* is primer3. > > My versions: > emboss-6.6.0 > primer3-2.3.5 > > Could you help me out? > > _______________________________________________ > EMBOSS mailing list > [email protected] > http://mailman.open-bio.org/mailman/listinfo/emboss
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