From: Peiser Benny <[EMAIL PROTECTED]> To: cambridge-conference <[EMAIL PROTECTED]> Subject: CCNet, 27 November 2000 Date: Mon, 27 Nov 2000 11:43:27 -0000 X-Mailer: Internet Mail Service (5.5.2650.21) CCNet 122/2000 - 27 November 2000 --------------------------------- "In like manner as a tree sheds its seed into the neighbouring fields and produces other trees; so the great vegetable, the world, or this planetary system, produces within itself certain seeds which, being scattered into the surrounding chaos, vegetate into new worlds. A comet, for instance, is the seed of a world; and after it has been fully ripened, by passing from sun to sun, and star to star, it is, at last, tossed into the uniformed elements which everywhere surround this universe, and immediately sprouts up into a new system." -- David Hume, Dialogues Concerning Natural Religion, 1779 "Still, a compelling case can be made for panspermia. A recent discovery indicates that microbes can remain dormant for millions of years -- enough time to travel from planet to planet. An experiment suggests that microbes inside a meteor would not be incinerated during entry into the Earth's atmosphere. While NASA's astrobiology effort has certainly not come down on the side of panspermia, it has identified panspermia as worthy of serious investigation, along with more conventional ideas about the origin of life on Earth." -- NASA Ames Research Centre, 22 November 2000 (1) SCIENTISTS CLAIM DISCOVERY OF MICROBE FROM SPACE CNN, 24 November 2000 (2) SCIENTIST'S EXTRAORDINARY CLAIM: ALIEN MICROBE DISCOVERED NASA Ames Research Center, 22 November 2000 (3) SIGNATURE OF POSSIBLE EXTRATERRESTRIAL BACTERIA AT 83KM IN THE ATMOSPHERE Cardiff Centre for Astrobiology, 24 November 2000 (4) A BACTERIAL "FINGERPRINT" IN A LEONID METEOR TRAIN Cardiff Centre for Astrobiology, 24 November 2000 (5) AN ATMOPSHERIC TEST OF COMETARY PANSPERMIA http://www.panspermia.org/balloon2.htm (6) THE PANSPERMIA THEORY ACCORDING TO HOYLE & WICKRAMASINGHE Chandra Wickramasinghe =============== (1) SCIENTISTS CLAIM DISCOVERY OF MICROBE FROM SPACE >From CNN, 24 November 2000 http://www.cnn.com/2000/TECH/space/11/24/alien.microbe.claim/index.html By Richard Stenger CNN.com Writer (CNN) -- An international team of scientists claims it has recovered a microorganism in the upper reaches of the atmosphere that originated from outer space. The living bacteria, plucked from an altitude of 10 miles (16 km) by a scientific balloon, could have been deposited in terrestrial airspace by a passing comet, according to the researchers. Noted scientist Chandra Wickramasinghe, a participant in the study, said the microbe is unlike any known strain on Earth. The astrobiology team recovered the microorganism samples from different heights for about a year, but "want to keep the details under wraps until they are absolutely convinced that these are extraterrestrial," said Wickramasinghe, a professor at Cardiff University in Wales. NASA's Ames Research Center posted a cautious reaction to the report on its Astrobiology Web site. NASA said the finding is likely to meet considerable skepticism in the scientific community. "Aerobiologists might argue that 10 miles is not too high for Earth life to reside, a possibility that Wickramasinghe appears to accept," the statement said. However, NASA said, a compelling case can be made for the transport of microorganisms through space aboard comets and meteors. "A recent discovery indicates that microbes can remain dormant for millions of years -- enough time to travel from planet to planet," NASA said. Disputing critics who suggest that the balloon was contaminated on the ground, Wickramasinghe said the experiment took place with strict controls. He does acknowledge the possibility that terrestrial bacteria could be kicked up into the stratosphere. Living fungal spores have been discovered at altitudes of 7 miles (11 km). But observations from this and a related study suggest the presence of living bacteria far too high in the atmosphere to have originated from the surface of the planet, according to Wickramasinghe. "What is present in the upper atmosphere, critics will say it came from the ground. That is a serious possibility at 15 kilometers, but at 40 or 85 kilometers, you can forget about it," he said Friday. Wickramasinghe and colleague Sir Fred Hoyle published a draft report on the Cardiff University Web site Friday about evidence that they say strengthens the hypothesis that unusual microbes float through the upper reaches of the atmosphere. Looking at spectral data from the 1999 Leonid meteorite shower, they detected a bacterial "fingerprint" as the tiny space rocks streaked across the sky. In other words, the micrometeorites burned through the atmospheric edge in a manner that suggests they sizzled microbes existing in the same airspace. "The bacteria heated at temperatures high enough to radiate and shine in this (spectral) signature," Wickramasinghe said. Along with Hoyle, Wickramasinghe pioneered "panspermia," the theory that outer space seeded Earth with its first life forms about 4 billion years ago. Wickramasinghe holds that primitive life could still be arriving from space. "If we find microbes at great heights that are not contaminants from the ground, we have to wonder where they came from. One hundred tons of comet and meteor organic debris is deposited in the atmosphere every day." Javant Narlikar of India lead the atmospheric bacteria sample study, which the Indian Space Research Organization coordinated. The location of the microbe is what most impressed Wickramasinghe, not the composition. It seems like a novel strain of a common bacteria genus on Earth, he said. Copyright 2000, CNN =============== (2) SCIENTIST'S EXTRAORDINARY CLAIM: ALIEN MICROBE DISCOVERED >From NASA Ames Research Center, 22 November 2000 http://astrobiology.arc.nasa.gov/news/expandnews.cfm?id=613 According to a report published in the London Daily Mail and picked up by United Press International, noted scientist Chandra Wickramasinghe claims to have found a microorganism not of this Earth. The microbe was collected by a weather balloon at a height of 10 miles. According to Wickramasinghe, the microorganism is unlike any other on Earth. He believes that it may have been dropped by a passing comet. Over twenty years ago, Wickramasinghe and colleague Fred Hoyle proposed the concept of panspermia, the idea that microorganisms may travel aboard comets and meteors, seeding other planets with life. Doubtless many will react to Wickramasinghe's announcement with disbelief. Skeptics will suspect that the balloon was contaminated at ground level, though Wickramasinghe disputes this. Microbiologists may be quick to point out that a large portion of the Earth's microscopic species remain to be discovered, and in recent years a number of astonishing species "unlike any other" have been uncovered right here on Earth. Aerobiologists might argue that 10 miles is not too high for Earth life to reside, a possibility that Wickramasinghe appears to accept. Living fungal spores have previously been reported at altitudes over 36,000 feet (roughly seven miles). Indeed, if the organism is found to earthly in origin, the discovery will be an exciting example of the extreme conditions that life can endure. Still, a compelling case can be made for panspermia. A recent discovery indicates that microbes can remain dormant for millions of years -- enough time to travel from planet to planet. An experiment suggests that microbes inside a meteor would not be incinerated during entry into the Earth's atmosphere. While NASA's astrobiology effort has certainly not come down on the side of panspermia, it has identified panspermia as worthy of serious investigation, along with more conventional ideas about the origin of life on Earth. This is by no means the first time that scientists have claimed to find microorgansims from outer space. In 1996, a team of NASA scientists reported finding microscopic fossils in a meteorite from Mars. Four years later, the jury is still out The NASA team stands firmly behind its findings, while many other astrobiologists -- including some from NASA -- remain unconvinced. Debate and scrutiny aren't always pleasant, but they're always essential to science. Stay tuned to Astrobiology at NASA as this debate unfolds. =============== (3) SIGNATURE OF POSSIBLE EXTRATERRESTRIAL BACTERIA AT 83KM IN THE ATMOSPHERE >From Cardiff Centre for Astrobiology, 24 November 2000 http://www.astrobiology.cf.ac.uk/news.html An infrared spectrum of a persistent Leonid meteor train in the November 1999 Leonids shower published by Ray W. Russell at his colleagues (Special edition of Earth Moon and Planets, on Leonids) shows an infrared signature that is indistinguishable from that of a bacterium. Wickramasinghe and Hoyle (Preprint) have argued that this arises from cometary bacteria entering the mesophere that have been transiently heated to temperatures of 130 degrees Celsius by the energy of the fire ball. The fireball is estimated to have a mass of above 100 kg. The bacteria from which the signature is detected forms part of the 100 tonnes per day daily input into the Earth of cometary organic material. The 1999 Leonid meteor is pictured below: http://www.astrobiology.cf.ac.uk/news.html ============= (4) A BACTERIAL "FINGERPRINT" IN A LEONID METEOR TRAIN >From Cardiff Centre for Astrobiology http://www.astrobiology.cf.ac.uk/preprint.html Chandra Wickramasinghe and Fred Hoyle Cardiff Centre for Astrobiology School of Mathematics, Senghennydd Road PO Box 926, Cardiff CF2 4YH, UK E-mail: [EMAIL PROTECTED] Abstract A recently observed broad 3.4m m spectral "fingerprint" in a persistent Leonid meteor train at a height of 83km is likely to be due to emission of surrounding mesospheric bacteria heated by the passage of an incandescent fireball. FULL PAPER at http://www.astrobiology.cf.ac.uk/preprint.html ============= (5) AN ATMOPSHERIC TEST OF COMETARY PANSPERMIA >From http://www.panspermia.org/balloon2.htm by N.C. WICKRAMASINGHE, F. HOYLE School of Mathematics, Cardiff University, PO Box 926, Senghennydd Road, Cardiff CF2 4YH, UK E-mail: [EMAIL PROTECTED] and J.V. NARLIKAR Inter-University Centre for Astronomy and Astrophysics Post Bag 4, Ganeshkhind, Pune 411 077, India E-mail: [EMAIL PROTECTED] Abstract Experiments currently under way could settle once and for all the beleaguered question of the existence or otherwise of microbial life on comets. A program of research planned by Indian scientists under the auspices of the Indian Space Research Organisation (ISRO) seems set to preempt results that may be expected from NASA's Stardust Mission to Comet Wild-2. 1. Introduction A direct way to test the theory of panspermia is to examine a sample of cometary material under the microscope and search for cometary microorganisms (Hoyle and Wickramasinghe, 1981). Such thoughts are being currently voiced in the context of the launch of NASA's Stardust Mission (launch date February 6, 1999) as well as in relation to other space missions being discussed for the new millennium. There seems to be a growing sense of optimism about possibly resolving a longstanding scientific question: Do comets carry the seeds of life in the Universe? If by "seeds" one means prebiotic chemicals, an affirmative answer is already at hand from the wealth of remote sensing data that is available for Comet Halley (Kissel and Krueger, 1987; Wickramasinghe, 1993). To get further affirmation of this restricted position from a comet sample return would seem an extravagant use of resources to say the least. Until a couple of years ago a more literal interpretation of the word "seeds" to include viable microorganisms was fraught with prejudice. All the early arguments and evidence that two of the present authors had presented for cometary microorganisms (e.g. Hoyle and Wickramasinghe, 1981) had been consistently ignored. This situation changed quite dramatically in 1996 when a claim of microfossils in a Martian meteorite (ALH84001) came to be discussed (McKay et al, 1996). Almost instantly investigations of panspermia came to be elevated to the status of legitimate scientific inquiry. Unfortunately, however, this long overdue change of attitude may have come too late to have had an influence in the planning of experiments connected with the Stardust Mission. 2. Stardust Mission The main object of the Stardust Mission is to capture a sample of dust from the well-preserved Comet Wild-2 on January 2, 2004 and to return this material safely to Earth on January 15, 2006. The comet dust is to be captured in a "particle catcher" filled with aerogel, the lowest density material known to exist. The hope is that the aerogel would act as a soft landing cushion to slow particles from an initial relative speed of 6.1 km/s to rest fairly gently without significantly modifying original chemical structures. At the time of planning the package of experiments that was to go on Stardust the concept of microbes on comets was still considered heretical and so "way out" as not to merit serious investigation. No experiment was explicitly planned to search for viable microoganisms, as far as we are aware. It is not clear that the integrity and viability of a bacterial spore, for example, would be preserved after a crash into the gel. In these circumstances one may still hope for the intervention of serendipity. Discoveries in the year 2006 might have a greater bearing on the question of cometary life than Stardust's planners expected. 3. Early Balloon Experiments Historically the earliest experiments to search for microbes in the upper atmosphere using balloons were conducted in the early 1960's with the aim of determining the microbial content (if any) of the near space environment, presumably as a preparation for manned space flights. Although microbiological techniques at this time were primitive compared to what is available today there were already some dramatic indications of extraterrestrial microbes in air samples collected at altitudes of 30 km and above (Bruch, 1967; Lysenko, 1979). Positive detection of microorganisms at 130,000ft (39km) and a population density that increased with height pointed to a possible extraterrestrial source. However, the smallness of samples collected as well as uncertainties in experimental procedures did not lend much confidence to believe what was "found". In consequence this early program of work was not pursued beyond an initial stage. 4. Plans for Indian Balloon Experiment A series of balloon experiments using the most modern microbiological techniques is being planned by the Indian Space Research Organisation (ISRO) and the Inter-Universities Centre for Astronomy and Astrophysics, Pune (IUCAA), with collaborative UK links in Cardiff. This program is explicitly directed to testing cometary panspermia at a minute fraction of the cost of the Stardust Mission. It has been known for several decades that cometary dust is present at our very doorstep, and all that is needed is to collect such dust non-destructively and without biological contamination. The sample collections are to be made using a balloon-borne cryogenic pump comprised of many sterilised chambers fitted with valves and cooled to liquid neon temperatures. When the valves are open at predetermined heights ambient air is sucked into the cryogenically cooled chambers. Such air samples, that would include cometary aerosols, are to be recovered and will be subject to careful chemical and microbial examination under contaminant free conditions. The Principal Investigator of the project and overall co-ordinator is Professor J.V. Narlikar, Director of the Inter-University Centre for Astronomy and Astrophysics in Pune. He will be assisted in this task by Professor S. Ramadurai of the Tata Institute of Fundamantal Research in Mumbai. Other scientists in the team on the Indian side are as listed below: Professor P. Rajaratnam, ISRO, Bangalore will direct operations relating to the Cryosampler experiment Professor P.C. Agrawal of the Tata Institute of Fundamental Research (TIFR) Mumbai, and Professor SV. Damle of the National Centre for Radio Astrophysics (NCRA), Pune will be responsible for the logistics of the Balloon Facility support and supervision, Professor Shyam Lal of the Physical Research Laboratory (PRL) Ahmedabad will direct the vacuum baking of probes Professor P.M. Bhargava, Anveshna Consulting Services, Hyderabad will direct the sterilization programme and act as overall co-ordinator of the microbiological investigations. In the U.K. the collaborating scientists are Professors Sir Fred Hoyle, David Lloyd, N.C. Wickramasinghe (co-Principal Investigator) and Dr. Max K. Wallis. Sterilisation techniques that are to be used in both sample retrieval and experimental preparation will be expected to achieve levels of microbial sterility that can essentially eliminate even the presence of single contaminant microorganism. At the same time the use of fluorescent dyes sensitive to membrane potential would permit the detection of single viable cells in the collected samples. Professor David Lloyd of Cardiff University has used this latter technique successfully in a number of other applications, and it is hoped that satisfactory results could be obtained in the present instance as well. Professor Lloyd will act as Project Director of Biological Sciences in the UK. Because isotope ratios (C, O, and H) will differ between extraterrestrial and terrestrial bacterial material, isotope analyses are to be carried out with a view to identifying extraterrestrial bacteria. 5. Cost of Project and Additional Support Required Several of the operational components of the project are already in place in various Indian Research Institutes. For instance a prototype cryogenic sampler has been recently used by the ISRO-PRL Group in their investigation of greenhouse gases in the stratosphere (Shyam Lal et al 1996), and cosmic ray physicists at the Tata Institute have used balloon-launching facilities over several decades. It is expected that the Indian Government will bear the major cost of the project, which is estimated at about £150,000. A contribution of £25,000 is being sought from UK sources to facilitate the purchase of experimental components that require foreign exchange, which is precious to the Indian Government. The Cardiff based part of the program also needs funds to about the same extent (£25,000), towards which a grant of US$8000 has been awarded. Further grants to fund the remainder will be sought from PPARC and NERC. 6. Estimates of Microbial Counts in Collected Samples There have been various estimates of the total input of cometary debris to the Earth, which is mainly in the form of microscopic dust. A plausible daily average flux is given to be F=500 metric tonnes (Chyba et al, 1990) or about 5x103 g/s. Of this let us suppose that a fraction x is in the form of cometary bacteria. Under steady state conditions the downward flux of such bacterial particles must balance the rate of infall from space. This gives an equilibrium number density N (per unit volume) N » 1000 x/mvS litre-1 (1) Where m is the average mass of a cometary microorganism, v is the average speed of fall through the stratosphere at say a height of 30km and S is the surface area of the Earth ~ 5x1018 cm2 . Earlier estimates of N assumed an average bacterial radius of 5x10-5 cm (mass ~ 10-12 g) and a corresponding value of v ~ 0.1cm/s (Narlikar et al, 1998). There is now growing evidence to suggest that microorganisms in a non-vegetative, nutrient-starved condition have significantly smaller sizes (see pictures in Pflug, 1984; Pflug and Heinz, 1998; Hoyle et al, 1985). A value of radius a » 10-5 cm would appear to be an appropriate average value, from which we get a mass m of ~ 10-14 g and a corresponding terminal velocity at 30km of v=0.01cm/s (Kasten, 1968). Together with F = 5x103 g/s we thus obtain from (1) a volume density N » 10,000 x litre-1 (2) (This number could be still higher if nanobacteria of average radii ~ 10-6 cm are considered to be an important component of the cometary microbial flora (Folk and Lynch, 1998). Increases of N by factors of over 1000 would be possible in this case.) Although it is impossible to arrive at a fully reliable estimate for x , a value close to 0.01 could be justified. If one argues that the flux of organic dust in the outer coma of Comet Halley (as measured by space probes in 1986) is predominantly bacterial for particle masses of the order 10-14 g, the data of McDonnell et al (1986) could be interpreted to give a mass fraction of such particles of nearly 1%. Thus (2) yields N~ 1000 litre-1 . For an anticipated air sample equivalent to 50-100 litres at NTP the bacterial count according to these estimates could be as high as ~ 100,000. As noted earlier this would be well above the detection thresholds of the experiments being planned. The above considerations are valid for the average population density of bacterial particles. The number N could be significantly higher on occasions when the Earth crosses major cometary meteor streams such as the Leonids. Over several days of such crossings the value of N could be enhanced by factors of the order of thousands or tens of thousands. If balloon flights and sample collections are scheduled for days coinciding with such enhancement, the statistical significance of the collected data could also be correspondingly enhanced. 7. Concluding Remarks Whilst it is now possible to deal effectively with most problems relating to equipment and sample purity that had proved difficult in the past, an outstanding problem to be resolved concerns the separation of extraterrestrial and terrestrial bacteria in the stratospheric collections. Several independent criteria could be used to show that stratosphere contains a mixture of two such distinct components. The microbial density profile with altitude when it is accurately determined could lead to an initial diagnostic showing a combination of infalling and outflowing components. This could show up for instance in a U-shaped density curve with a definite minimum occurring at some altitude. Decisive evidence of an extraterrestrial bacterial component must, however, come from laboratory experiments. Microscopic studies may show distinctive morphologies that are either unknown or rare in a terrestrial environment. Biochemical studies including determinations of the D/L ratios of amino acid enantiomers could lead to further diagnostic and distinctive criteria being discovered. Isotopic analyses could also lead to decisive results in relation to the distribution of the C12 /C13 isotope ratio in terrestrial and extraterrestrial organisms. A positive detection of cometary microorganisms and a vindication of panspermia theory would obviously have far reaching scientific consequences. The existence of extraterrestrial life and its relationship to terrestrial life, once established, would surely prove a fitting finale to an eventful century of science. It would inevitably open the doorway to new scientific vistas, which it would be the privilege of future generations to explore. References Bruch, C.W.: 1967. Airborne Microbes Symposium of the Society for Microbiology. No. 17 (P.H. Gregory and J.L. Monteith, eds) p. 345, Cambridge University Press Chyba, C.F., Thomas, P.T., Brookshaw, L. and Sagan, C.: 1990. Science, 249, 366 Folk, R.L. and Lynch, F.L.: 1998. Proceedings of SPIE Conference on Instruments, Methods and Missions for Astrobiology, 3441, 112 Hoyle, F. and Wickramasinghe, N .C.: 1981. In Comets and the Origin of Life (C. Ponnamperuma, ed), D. Reidel Publishing Co. Hoyle, F., Wickramasinghe, N .C. and Pflug, H.D: 1985. Astrophys Sp Sci, 113, 209 Hoyle, F. and Wickramasinghe, N .C.: 1990. The Theory of Cosmic Grains, Kluwer, 1990 Kasten, F.J.: 1968. Appl. Meteorology, 7, 944 Kissel, J. and Krueger, F.R.: 1987. Nature, 326, 760 Mc Donnell, J.A.M. et al.: 1986. ESA-SP, 250 (2), 25 McKay, D.S. et al.: 1996. Science, 273, 924 Lysenko, S.V.: 1979. Mikrobiologia, 48, 1066 Narlikar, J.V. et al.: 1998. Proceedings of SPIE Conference on Instruments, Methods and Missions for Astrobiology, 3441, 301 Pflug, H.D.: 1984. in Fundamental Studies and the Future of Science (C. Wickramasinghe, ed), University College Cardiff Press Pflug, H.D. and Heinz, B.: 1998. Proceedings of SPIE Conference on Instruments, Methods and Missions for Astrobiology, 3441, 188 Shyam Lal et al.: 1996. Ind. J. Rad.Sp.Phys., 26, 1 Wickramasinghe, N .C.: 1993. In Infrared Astronomy (A. Mampaso, M. Prieto and F. Sanchez, eds), p.303, Cambridge University =========== (6) THE PANSPERMIA THEORY ACCORDING TO HOYLE & WICKRAMASINGHE >From Chandra Wickramasinghe http://www.wickramasinghe.freeserve.co.uk/ Fred Hoyle and Chandra Wickramasinghe began their work that eventually led to a new theory of Panspermia in 1974. In this year Chandra Wickramasinghe had discovered that interstellar dust might have a substantial component of organic polymers (Nature, 252,462,1974). Gradually the idea of biogenic material in space came to be developed, and in 1979-1982 the first strong evidence that almost all the interstellar dust may be made up of freeze-dried bacterial material was discovered (ApSS,66,77, 1979; ApSS, 83, 405,1982). MAIN POINTS OF THEORY * Life on Earth which first appeared about 4000 million years ago, at a time when the planet was being severely bombarded by comets and asteroids, could not have started on Earth * Life in the form of bacteria and viruses were brought to Earth by comets, which were the sites where they multiplied (in warm watery interiors), and which acted as vehicles of transport * Microorganisms continue to arrive at the Earth even today, being included in the 100 tons or so of cometary debris that enters the Earth on a daily basis * The continued arrival of cometary bacteria and viruses contributes to the evolution of species through geological time * Interactions of present day life forms on Earth (including) humans with cometary bacteria and viruses could lead to epidemic disease * Astronomical evidence is fully consistent with the occurrence of microorganisms on a cosmic scale...... ---------------------------------------------------------------------------- ------------------ THE CAMBRIDGE-CONFERENCE NETWORK (CCNet) ---------------------------------------------------------------------------- ------------------ The CCNet is a scholarly electronic network. 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