Hi folks. Our Exchange is down. I've been troubleshooting since yesterday.  Its 
SBS 2008 running Exchange 2007 on one server. All Exchange roles and DC/GC are 
on the same physical box.  Currently, the Transport service starts and then 
stops.
I am running a local nt backup to try and have a recovery point plus free or 
clear any transaction logs.

A little background:
Users started complaining yesterday about email access - they could send out, 
and send/recv internally, but no incoming mail was getting through.
I tried to use telnet from a remote computer to test smtp on the server.  When 
I connected via telnet, and did the helo, mail from:drkuhl...@yahoo.com I 
was getting the "insufficient resources 4.3.2" message
I did a shutdown and restart on the server
No luck.

So I started googling and found some blurbs about modifying the 
edgetransport.exe.config file and setting "EnableResourceMonitoring" 
value="false" to see if things got better while working to free up resources 
and stop what I think is a "back pressure" situation.

Since that didn't help, I put the backup copy of the edgetransport.exe.config 
file back in place.

Now getting "connection refused" with the transport service not being able to 
stay started.  And we can't send/recv internally or externally now.
I don't know if the service issues is currently because of the backup running 
but wanted to get some feelers out to try and work on later after it finishes 
if I still can't get the service to run.

The event attached below seems to indicate that a file is locked or in use, 
maybe because of the backup running?

Any help or pointers would be appreciated.

Thanks

Don K

Here is a snip of one event relating to the transport service failure.

Log Name:      Application

Source:        MSExchangeTransport
Date:          6/7/2012 2:36:17 PM
Event ID:      7001
Task Category: Components
Level:         Information
Keywords:      Classic
User:          N/A
Computer:      SMISBS010.corp.abtrex.com
Description:
The service will be stopped. Reason: 
Microsoft.Exchange.Transport.TransportComponentLoadFailedException: Loading of 
component 'AD Configuration Readers' failed. ---> 
Microsoft.Exchange.Transport.TransportComponentLoadFailedException: The 
transport component 'SequentialTransportComponent' couldn't be loaded 
successfully. ---> 
Microsoft.Exchange.Transport.TransportComponentLoadFailedException: Loading of 
component 'Database' failed. ---> 
Microsoft.Exchange.Transport.TransportComponentLoadFailedException: The 
following database is in use: Queuing Database. ---> 
Microsoft.Exchange.Isam.IsamFileAccessDeniedException: Cannot access file, the 
file is locked or in use (-1032)
   at Microsoft.Exchange.Isam.?A0xf73de38c.HandleError(Int32 err)
   at Microsoft.Exchange.Isam.Interop.MJetInit(MJET_INSTANCE instance)
   at Microsoft.Exchange.Transport.Storage.DataSource.InitInstance()
   at Microsoft.Exchange.Transport.Storage.DataSource.OpenDatabase()
   at 
Microsoft.Exchange.Transport.Storage.Messaging.Database.AttachInternal(String 
path, Boolean wipe)
   at Microsoft.Exchange.Transport.Storage.Messaging.Database.Attach()
   --- End of inner exception stack trace ---
   at Microsoft.Exchange.Transport.Storage.Messaging.Database.Attach()
   at 
Microsoft.Exchange.Transport.SequentialTransportComponent.<>c__DisplayClass2.<Load>b__0()
   at 
Microsoft.Exchange.Data.Directory.ADNotificationAdapter.RunADOperation(ADOperation
 adOperation, Int32 retryCount)
   at 
Microsoft.Exchange.Data.Directory.ADNotificationAdapter.TryRunADOperation(ADOperation
 adOperation, Int32 retryCount)
   at Microsoft.Exchange.Transport.SequentialTransportComponent.Load()
   --- End of inner exception stack trace ---
   at Microsoft.Exchange.Transport.SequentialTransportComponent.Load()
   at 
Microsoft.Exchange.Transport.ParallelTransportComponent.<>c__DisplayClass2.<OnLoadChild>b__0()
   at 
Microsoft.Exchange.Data.Directory.ADNotificationAdapter.RunADOperation(ADOperation
 adOperation, Int32 retryCount)
   at 
Microsoft.Exchange.Data.Directory.ADNotificationAdapter.TryRunADOperation(ADOperation
 adOperation, Int32 retryCount)
   at 
Microsoft.Exchange.Transport.ParallelTransportComponent.OnLoadChild(Object 
state)
   --- End of inner exception stack trace ---
   at Microsoft.Exchange.Transport.ParallelTransportComponent.Load()
   at 
Microsoft.Exchange.Transport.SequentialTransportComponent.<>c__DisplayClass2.<Load>b__0()
   at 
Microsoft.Exchange.Data.Directory.ADNotificationAdapter.RunADOperation(ADOperation
 adOperation, Int32 retryCount)
   at 
Microsoft.Exchange.Data.Directory.ADNotificationAdapter.TryRunADOperation(ADOperation
 adOperation, Int32 retryCount)
   at Microsoft.Exchange.Transport.SequentialTransportComponent.Load()
   --- End of inner exception stack trace ---
   at Microsoft.Exchange.Transport.SequentialTransportComponent.Load()
   at Microsoft.Exchange.Transport.Components.Activate()
Event Xml:
<Event xmlns="http://schemas.microsoft.com/win/2004/08/events/event";>
  <System>
    <Provider Name="MSExchangeTransport" />
    <EventID Qualifiers="16388">7001</EventID>
    <Level>4</Level>
    <Task>6</Task>
    <Keywords>0x80000000000000</Keywords>
    <TimeCreated SystemTime="2012-06-07T18:36:17.000Z" />
    <EventRecordID>486390</EventRecordID>
    <Channel>Application</Channel>
    <Computer>SMISBS010.corp.abtrex.com</Computer>
    <Security />
  </System>
  <EventData>
    <Data>Microsoft.Exchange.Transport.TransportComponentLoadFailedException: 
Loading of component 'AD Configuration Readers' failed. ---&gt; 
Microsoft.Exchange.Transport.TransportComponentLoadFailedException: The 
transport component 'SequentialTransportComponent' couldn't be loaded 
successfully. ---&gt; 
Microsoft.Exchange.Transport.TransportComponentLoadFailedException: Loading of 
component 'Database' failed. ---&gt; 
Microsoft.Exchange.Transport.TransportComponentLoadFailedException: The 
following database is in use: Queuing Database. ---&gt; 
Microsoft.Exchange.Isam.IsamFileAccessDeniedException: Cannot access file, the 
file is locked or in use (-1032)
   at Microsoft.Exchange.Isam.?A0xf73de38c.HandleError(Int32 err)
   at Microsoft.Exchange.Isam.Interop.MJetInit(MJET_INSTANCE instance)
   at Microsoft.Exchange.Transport.Storage.DataSource.InitInstance()
   at Microsoft.Exchange.Transport.Storage.DataSource.OpenDatabase()
   at 
Microsoft.Exchange.Transport.Storage.Messaging.Database.AttachInternal(String 
path, Boolean wipe)
   at Microsoft.Exchange.Transport.Storage.Messaging.Database.Attach()
   --- End of inner exception stack trace ---
   at Microsoft.Exchange.Transport.Storage.Messaging.Database.Attach()
   at 
Microsoft.Exchange.Transport.SequentialTransportComponent.&lt;&gt;c__DisplayClass2.&lt;Load&gt;b__0()
   at 
Microsoft.Exchange.Data.Directory.ADNotificationAdapter.RunADOperation(ADOperation
 adOperation, Int32 retryCount)
   at 
Microsoft.Exchange.Data.Directory.ADNotificationAdapter.TryRunADOperation(ADOperation
 adOperation, Int32 retryCount)
   at Microsoft.Exchange.Transport.SequentialTransportComponent.Load()
   --- End of inner exception stack trace ---
   at Microsoft.Exchange.Transport.SequentialTransportComponent.Load()
   at 
Microsoft.Exchange.Transport.ParallelTransportComponent.&lt;&gt;c__DisplayClass2.&lt;OnLoadChild&gt;b__0()
   at 
Microsoft.Exchange.Data.Directory.ADNotificationAdapter.RunADOperation(ADOperation
 adOperation, Int32 retryCount)
   at 
Microsoft.Exchange.Data.Directory.ADNotificationAdapter.TryRunADOperation(ADOperation
 adOperation, Int32 retryCount)
   at 
Microsoft.Exchange.Transport.ParallelTransportComponent.OnLoadChild(Object 
state)
   --- End of inner exception stack trace ---
   at Microsoft.Exchange.Transport.ParallelTransportComponent.Load()
   at 
Microsoft.Exchange.Transport.SequentialTransportComponent.&lt;&gt;c__DisplayClass2.&lt;Load&gt;b__0()
   at 
Microsoft.Exchange.Data.Directory.ADNotificationAdapter.RunADOperation(ADOperation
 adOperation, Int32 retryCount)
   at 
Microsoft.Exchange.Data.Directory.ADNotificationAdapter.TryRunADOperation(ADOperation
 adOperation, Int32 retryCount)
   at Microsoft.Exchange.Transport.SequentialTransportComponent.Load()
   --- End of inner exception stack trace ---
   at Microsoft.Exchange.Transport.SequentialTransportComponent.Load()
   at Microsoft.Exchange.Transport.Components.Activate()</Data>
  </EventData>
</Event>
---
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